解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::RNA editing in the mitochondria of kinetoplastids involves the addition and deletion of uridines at specific sites as directed by guide RNAs (gRNAs). Ample evidence shows that ribonucleoprotein (RNP) complexes carry out this posttranscriptional processing. One component of RNA editing complexes is REAP-1, a protein of...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00231-3
更新日期:2001-04-01 00:00:00
abstract::Growth factors such as epidermal growth factor (EGF) and insulin regulate development and metabolism via genes containing both POU homeodomain (Pit-1) and phorbol ester (AP-1) response elements. Although CREB binding protein (CBP) functions as a coactivator on these elements, the mechanism of transactivation was previ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00202-7
更新日期:2001-03-01 00:00:00
abstract::Clathrin-dependent endocytosis has long been presented as the only efficient mechanism by which transmembrane receptors are internalized. We selectively blocked this process using dominant-negative mutants of Eps15 and showed that clathrin-mediated endocytosis of transferrin was inhibited, while endocytosis of interle...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00212-x
更新日期:2001-03-01 00:00:00
abstract::Nhp6A and Nhp6B are HMG1-like proteins required for the growth of S. cerevisiae at elevated temperatures. We show that the conditional lethality of an nhp6 strain results from defective transcription of SNR6 (U6 snRNA) by RNA polymerase III. Overexpression of U6 snRNA or Brf1, a limiting component of TFIIIB, and an ac...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00179-4
更新日期:2001-02-01 00:00:00
abstract::Early endocytic membrane traffic is regulated by the small GTPase Rab5, which cycles between GTP- and GDP-bound states as well as between membrane and cytosol. The latter cycle depends on GDI, which functions as a Rab vehicle in the aqueous environment of the cytosol. Here, we report that formation of the GDI:Rab5 com...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00189-7
更新日期:2001-02-01 00:00:00
abstract::Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through a...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00153-8
更新日期:2001-01-01 00:00:00
abstract::How repeating striped patterns arise across cellular fields is unclear. To address this we examined the repeating pattern of Stripe (Sr) expression across the parasegment (PS) in Drosophila. This pattern is generated in two steps. First, the ligands Hedgehog (Hh) and Wingless (Wg) subdivide the PS into smaller territo...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(01)00163-0
更新日期:2001-01-01 00:00:00
abstract::Ubiquitin-mediated proteolysis regulates the activity of diverse receptor systems. Here, we identify Smurf2, a C2-WW-HECT domain ubiquitin ligase and show that Smurf2 associates constitutively with Smad7. Smurf2 is nuclear, but binding to Smad7 induces export and recruitment to the activated TGF beta receptor, where i...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00134-9
更新日期:2000-12-01 00:00:00
abstract::AAA ATPases play central roles in cellular activities. The ATPase p97, a prototype of this superfamily, participates in organelle membrane fusion. Cryoelectron microscopy and single-particle analysis revealed that a major conformational change of p97 during the ATPase cycle occurred upon nucleotide binding and not dur...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00144-1
更新日期:2000-12-01 00:00:00
abstract::There are few tools available for dissecting and elucidating the functions of DNA satellites and other nongenic DNA. To address this, we have explored the experimental potential of DNA sequence-specific drugs containing pyrrole and imidazole amino acids (polyamides). Compounds were synthesized that target different Dr...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00099-x
更新日期:2000-11-01 00:00:00
abstract::JunD is the most broadly expressed member of the Jun family and the AP-1 transcription factor complex. Primary fibroblasts lacking JunD displayed p53-dependent growth arrest, upregulated p19(Arf) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00109-x
更新日期:2000-11-01 00:00:00
abstract::Perfringolysin O, a bacterial cytolytic toxin, forms unusually large pores in cholesterol-containing membranes by the spontaneous insertion of two of its four domains into the bilayer. By monitoring the kinetics of domain-specific conformational changes and pore formation using fluorescence spectroscopy, the temporal ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00119-2
更新日期:2000-11-01 00:00:00
abstract::The Holliday junction is a central intermediate in homologous recombination. It consists of a four-way structure that can be resolved by cleavage to give either the crossover or noncrossover products observed. We show here that the formation of these products is controlled by the E. coli resolvasome (RuvABC) in such w...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(05)00095-x
更新日期:2000-10-01 00:00:00
abstract::We show that the IRF-2 oncoprotein represses virus-induced IFN-beta gene transcription via a novel mechanism. Virus infection induces recruitment of IRF-2 to some of the endogenous IFN-beta enhancers as part of the enhanceosome. Enhanceosomes bearing IRF-2 cannot activate transcription, due to the presence of a domain...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(05)00081-x
更新日期:2000-10-01 00:00:00
abstract::We used DNA microarrays to profile gene expression patterns in the C. elegans germline and identified 1416 germline-enriched transcripts that define three groups. The sperm-enriched group contains an unusually large number of protein kinases and phosphatases. The oocyte-enriched group includes potentially new componen...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00059-9
更新日期:2000-09-01 00:00:00
abstract::Recently, a novel family of TATA binding protein (TBP)-like factors (TLFs) have been described in metazoan organisms; however, their function has not yet been elucidated. Using Caenorhabditis elegans (Ce) as a model, we demonstrate that CeTLF is required in vivo for zygotic transcription during embryogenesis. Eliminat...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00069-1
更新日期:2000-09-01 00:00:00
abstract::Baculoviral IAP repeat proteins (BIRPs) may affect cell death, cell division, and tumorigenesis. The C. elegans BIRP BIR-1 was localized to chromosomes and to the spindle midzone. Embryos and fertilized oocytes lacking BIR-1 had defects in chromosome behavior, spindle midzone formation, and cytokinesis. We observed in...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00023-x
更新日期:2000-08-01 00:00:00
abstract::Three introns whose splicing is activated during meiosis in S. cerevisiae contain a Mer1p-dependent splicing enhancer. The enhancer can impose Mer1p-activated splicing upon the constitutively spliced actin intron provided the basal splicing efficiency of actin is first reduced. Of several nonessential splicing factors...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00033-2
更新日期:2000-08-01 00:00:00
abstract::The CCCH finger protein PIE-1 is a regulator of germ cell fate that segregates with the germ lineage in early embryos. At each asymmetric division, PIE-1 is inherited preferentially by the germline daughter and is excluded from the somatic daughter. We show that this asymmetry is regulated at the protein level by two ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)00043-5
更新日期:2000-08-01 00:00:00
abstract::The liver plays a central role in the control of glucose homeostasis and is subject to complex regulation by substrates, insulin, and other hormones. To investigate the effect of the loss of direct insulin action in liver, we have used the Cre-loxP system to inactivate the insulin receptor gene in hepatocytes. Liver-s...
journal_title:Molecular cell
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::The mRNA cap structure is bound by either the nuclear (CBC) or the cytoplasmic (eIF4F) cap binding complex. Following mRNA export, CBC must be exchanged for eIF4F in the cytoplasm. It is not known how this exchange occurs or how this RNP remodeling event is integrated with mRNA function. Here we report genetic and bio...
journal_title:Molecular cell
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::The mechanism by which (CTG)n expansion in the 3' UTR of the DMPK gene causes myotonic dystrophy (DM) is unknown. We identified four RNA splicing factors--hnRNP C, U2AF (U2 auxiliary factor), PTB (polypyrimidine tract binding protein), and PSF (PTB associated splicing factor)--that bind to two short regions 3' of the ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80261-0
更新日期:2000-06-01 00:00:00
abstract::Gene-targeted knockout mice have been generated lacking the major uracil-DNA glycosylase, UNG. In contrast to ung- mutants of bacteria and yeast, such mice do not exhibit a greatly increased spontaneous mutation frequency. However, there is only slow removal of uracil from misincorporated dUMP in isolated ung-/- nucle...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80271-3
更新日期:2000-06-01 00:00:00
abstract::Formation and maintenance of specialized plasma membrane domains are crucial for many biological processes, such as cell polarization and signaling. During isotropic bud growth, the yeast cell periphery is divided into two domains: the bud surface, an active site of exocytosis and growth, and the relatively quiescent ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80324-x
更新日期:2000-05-01 00:00:00
abstract::ClpXP is a protein machine composed of the ClpX ATPase, a member of the Clp/Hsp100 family of remodeling enzymes, and the ClpP peptidase. Here, ClpX and ClpXP are shown to catalyze denaturation of GFP modified with an ssrA degradation tag. ClpX translocates this denatured protein into the proteolytic chamber of ClpP an...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80243-9
更新日期:2000-04-01 00:00:00
abstract::CREB-binding protein (CBP) possesses an intrinsic acetyltransferase activity capable of acetylating nucleosomal histones as well as several nonhistone proteins. Here, it is shown that CBP can acetylate hepatocyte nuclear factor-4 (HNF-4), a member of the nuclear hormone receptor family, at lysine residues within the n...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80253-1
更新日期:2000-04-01 00:00:00
abstract::The steroid hormone ecdysone signals the stage-specific programmed cell death of the larval salivary glands during Drosophila metamorphosis. This response is preceded by an ecdysone-triggered switch in gene expression in which the diap2 death inhibitor is repressed and the reaper (rpr) and head involution defective (h...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80439-6
更新日期:2000-03-01 00:00:00
abstract::The papillomavirus major late protein, L1, forms the pentameric assembly unit of the viral shell. Recombinant HPV16 L1 pentamers assemble in vitro into capsid-like structures, and truncation of ten N-terminal residues leads to a homogeneous preparation of 12-pentamer, icosahedral particles. X-ray crystallographic anal...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80449-9
更新日期:2000-03-01 00:00:00
abstract::The crystal structure of a heterodimer between the ligand-binding domains (LBDs) of the human RARalpha bound to a selective antagonist and the constitutively active mouse RXRalphaF318A mutant shows that, pushed by a bulky extension of the ligand, RARalpha helix H12 adopts an antagonist position. The unexpected presenc...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80424-4
更新日期:2000-02-01 00:00:00
abstract::Bicoid (BCD), the anterior determinant of Drosophila, controls embryonic gene expression by transcriptional activation and translational repression. Both functions require the homeodomain (HD), which recognizes DNA motifs at target gene enhancers and a specific sequence interval in the 3' untranslated region of caudal...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80434-7
更新日期:2000-02-01 00:00:00
abstract::During meiosis, the homologous chromosomes pair and recombine. An evolutionarily conserved protein structure, the synaptonemal complex (SC), is located along the paired meiotic chromosomes. We have studied the function of a structural component in the axial/lateral element of the SC, the synaptonemal complex protein 3...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80404-9
更新日期:2000-01-01 00:00:00
abstract::The Drosophila gene vasa (vas) encodes an RNA-binding protein required for embryonic patterning and germ cell specification. In vas mutants, translation of several germline mRNAs is reduced. Here we show that VAS interacts directly with the Drosophila homolog of yeast translation initiation factor 2, encoded by a nove...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80414-1
更新日期:2000-01-01 00:00:00
abstract::In Xer site-specific recombination, sequential DNA strand exchange reactions are catalyzed by a heterotetrameric complex composed of two recombinases, XerC and XerD. It is demonstrated that XerC and XerD catalytic activity is controlled by an interaction involving the C-terminal end of each protein (the donor region) ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80224-5
更新日期:1999-12-01 00:00:00
abstract::The L. monocytogenes protein lnlB activates phosphoinositide 3-kinase and induces phagocytosis in several mammalian cell types. The 1.86 A resolution X-ray crystal structure of the leucine-rich repeat domain of lnlB that is both necessary and sufficient to induce phagocytosis is presented here. The structure supports ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80234-8
更新日期:1999-12-01 00:00:00
abstract::p300/CBP and PCAF coactivators have acetyltransferase activities and regulate transcription, cell cycle progression, and differentiation. They are both required for MyoD activity and muscle differentiation. Nevertheless, their roles must be different since the acetyltransferase activity of PCAF but not of p300 is invo...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80383-4
更新日期:1999-11-01 00:00:00
abstract::The mechanisms controlling growth and patterning along the proximal-distal axis of the vertebrate limb are yet to be understood. We show that restriction of expression of the homeobox gene Meis2 to proximal regions of the limb bud is essential for limb development, since ectopic Meis2 severely disrupts limb outgrowth....
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80393-7
更新日期:1999-11-01 00:00:00
abstract::Deep orange and carnation are two of the classic eye color genes in Drosophila. Here, we demonstrate that Deep orange is part of a protein complex that localizes to endosomal compartments. A second component of this complex is Carnation, a homolog of Sec1p-like regulators of membrane fusion. Because complete loss of d...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80199-9
更新日期:1999-10-01 00:00:00
abstract::The nuclear hormone receptor PPAR gamma promotes adipogenesis and macrophage differentiation and is a primary pharmacological target in the treatment of type II diabetes. Here, we show that PPAR gamma gene knockout results in two independent lethal phases. Initially, PPAR gamma deficiency interferes with terminal diff...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80209-9
更新日期:1999-10-01 00:00:00
abstract::Mammalian lefty and zebrafish antivin form a subgroup of the TGF beta superfamily. We report that mouse mutants for lefty2 have an expanded primitive streak and form excess mesoderm, a phenotype opposite to that of mutants for the TGF beta gene nodal. Analogously, overexpression of Antivin or Lefty2 in zebrafish embry...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80331-7
更新日期:1999-09-01 00:00:00
abstract::In eukaryotes, the 20S proteasome contains two chymotrypsin-like, two trypsin-like, and two active sites shown here to have caspase-like specificity. We report that certain sites allosterically regulate each other's activities. Substrates of a chymotrypsin-like site stimulate dramatically the caspase-like activity and...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(00)80341-x
更新日期:1999-09-01 00:00:00